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Computational Biophysics & Bioinformatics - Dr. Emil Alexov Group
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SAAMBE Web Server

Single Amino Acid Mutation related change of Binding Energy (SAAMBE) method addresses the demand for computational tools of predicting the effect of single amino acid substitution on the binding free energy of protein complexes. It is based on the fast (<< 1 minute) modified MM-PBSA protocol that is successfully tested and optimized for more than thousand experimental data points.

If the usage of the server results in scientific publication, please, cite the following papers: References

SAAMBE is running on Clemson University's Palmetto Supercomputer Cluster. If you experience problems, they may be due to Palmetto Cluster being not functional or under maintenance. Contact us at

Click on the following links to obtain a sample PDB file along with instructions of selecting parameter and predicted results.
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Original Amino Acid:
Mutated Amino Acid:

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