Requirement:
Python>=3
xgboost==0.82

Available command-line options:
  -A protein sequence
  -p Resid of the mutation
  -w One letter of wild amino acid
  -m One letter of mutant amino acid
  -f Input file
  -o Output file
  -h Display this command-line summary
  

1. blast software can copy to any directory.
2. Please change the installation directory of blast and protein reference database(nr database recommend) in the python script.
3. run the python script:
 1) single mutation
 python Mutation_pred_standard.py -A protein_sequence -p position -w wild -m mutant -o outputfile(Optional)
 2) mutations list
 python Mutation_pred_standard.py -A protein_sequence -f mutation_list.txt -o outputfile(Optional)

Example:
python Mutation_pred_standard.py -A fastaA -p 182 -w C -m A
python Mutation_pred_standard.py -A fastaA -f mutation_list.txt